Registration now closed.
We are inviting 12 members of the neuroscience community to join us for a 4-day course on our campus in Ann Arbor from May 21-24. The MINT Workshop will be heavily subsidized by the NSF. Participants will only be responsible for the transportation costs and their dinners. Registration and supplies are graciously provided by NSF NeuroNex.
Registration will close on March 16, 2018. If demand exceeds the class size then positions will be selected by the instructors based on the applicants prior experience and intended usage. Please briefly describe your experience and broad experimental use (no confidential details please). Our shared vision is to disseminate as many of these technologies conjointly for the acceleration of neuroscience. A final decision will be emailed to all by March 23, 2018.
- Theory and practice of tissue clearing (brain and skull) using CLARITY and variants
- Label of tissue in conjunction with tissue clearing, including IHC, protein, and RNA labeling
- Theory and hands-on imaging of tissue cleared samples, including 1 hr of confocal time
- Theory and practice of AAVs that can be delivered systemically with novel caspids, e.g. AAV-PHP.B and -PHP.eB
Instructor: Dawen Cai
- Principles of stochastic multispectral labeling of the nervous system
- Understand Brainbow sample preparation
- Theory and practice of Brainbow 3D imaging, including 2 or 3 hrs of confocal time
- Post-acquisition image processing and neuronal tracing of Brainbow images
- Prerequisites: stereotactic surgery
- Hands-on optoelectrode preparation for chronic surgery (designing and using microdrives)
- Methods with optoelectrodes, including cell-type identification and noise/artifact reduction
- Hands-on surgical experience using a chronic uLED array with simultaneous multi-channel stimulation and recording
- Understanding of high-density electrode recording/stimulation arrays including electrochemical impedance spectroscopy, cyclic voltammetry, and electrode materials
- Hands-on characterization of high surface area platinum electrodes, including charge balanced stimulation techniques
- Review of software options for high-density polytrode spike sorting
Note: The training workshop is intended for experienced graduate students, or postdocs and PIs. Please send questions to ContactMINT@umich.edu.