Workshop

Application is now CLOSED.  Please stay tuned for next Workshop. 

 

We are inviting approximately 20 members of the neuroscience community to join us for a 5-day course on our campus in Ann Arbor from June 17-21, 2019. The MINT Workshop will be heavily subsidized by the NSF. Participants will only be responsible for the transportation costs and their dinners. Registration and supplies are graciously provided by NSF NeuroNex. We will inform all applicants on or before May 20th whether there was space for accommodation. 

Download a PDF of the preliminary 2019 MINT Workshop Summary by Track. 

At the workshop, you can select 1 of 2 tracks, either Optoelectrode/ePhys (physiological) or Brainbow/Clarity/AAV (anatomical). See the workshop descriptions below for learning objectives and select your preferred track in the application.

1. Optogenetic Experimentation Using a uLED Optoelectrode

Instructors: Kamran Diba. James Weiland, John Seymour, Paras Patel

Lesson goals:

  • Prerequisites: stereotactic surgery
  • Hands-on optoelectrode preparation for chronic surgery (designing and using microdrives)
  • Methods with optoelectrodes, including cell-type identification and noise/artifact reduction
  • Hands-on surgical experience using a chronic uLED array with simultaneous multi-channel stimulation and recording

Lectures include:

  • Understanding of high-density electrode recording/stimulation arrays including electrochemical impedance spectroscopy, cyclic voltammetry, and electrode materials
  • Hands-on characterization of high surface area platinum electrodes, including charge balanced stimulation techniques
  • Review of software options for high-density polytrode spike sorting

Note: The training workshop is intended for experienced graduate students, or postdocs and PIs. Please send questions to ContactMINT@umich.edu

 

2. Reconstructing neural circuits by labeling, imaging, Clarity, and tracing Brainbow samples

Instructor: Dawen Cai, Viviana Gradinaru

Lesson goals:

  • Theory and practice of tissue clearing (brain and skull) using CLARITY and variants
  • Label of tissue in conjunction with tissue clearing, including IHC, protein, and RNA labeling
  • Theory and hands-on imaging of tissue cleared samples, including 1 hr of confocal time
  • Clarity techniques and expansion microscopy for high resolution imaging

Lectures include:

  • Theory and practice of AAVs, including systemic AAVs PHP.eB and PHP.S