Workshop

We are inviting approximately 20 members of the neuroscience community to join us for a 5-day course on our campus in Ann Arbor, Michigan from May 11th-15th, 2020. 

The MINT Workshop will be heavily subsidized by the NSF. Participants will only be responsible for the transportation costs and their dinners. Registration and supplies are graciously provided by NSF NeuroNex

Applications are due no later than January 24th, 2020.  We expect to inform all applicants by February 17th, 2020 whether there was space for accommodation. 

Application for 2020 is now OPEN.

Please select 1 of 2 tracks, either Optoelectrode/ePhys (physiological) or Brainbow/Clarity/AAV (anatomical) in your application.  See the workshop descriptions below for learning objectives.

Please email ContactMINT@umich.edu with questions.

1. Optogenetic Experimentation Using a uLED Optoelectrode

Instructors: Kamran Diba. James Weiland, John Seymour, Paras Patel

Lesson goals:

  • Prerequisites: stereotactic surgery
  • Hands-on optoelectrode preparation for chronic surgery (designing and using microdrives)
  • Methods with optoelectrodes, including cell-type identification and noise/artifact reduction
  • Hands-on surgical experience using a chronic uLED array with simultaneous multi-channel stimulation and recording

Lectures include:

  • Understanding of high-density electrode recording/stimulation arrays including electrochemical impedance spectroscopy, cyclic voltammetry, and electrode materials
  • Hands-on characterization of high surface area platinum electrodes, including charge balanced stimulation techniques
  • Review of software options for high-density polytrode spike sorting

Note: The training workshop is intended for experienced graduate students, or postdocs and PIs. Please send questions to ContactMINT@umich.edu

 

2. Reconstructing neural circuits by labeling, imaging, Clarity, and tracing Brainbow samples

Instructor: Dawen Cai, Viviana Gradinaru

Lesson goals:

  • Theory and practice of tissue clearing (brain and skull) using CLARITY and variants
  • Label of tissue in conjunction with tissue clearing, including IHC, protein, and RNA labeling
  • Theory and hands-on imaging of tissue cleared samples, including 1 hr of confocal time
  • Clarity techniques and expansion microscopy for high resolution imaging

Lectures include:

  • Theory and practice of AAVs, including systemic AAVs PHP.eB and PHP.S