Workshop

Registration now closed.

We are inviting 12 members of the neuroscience community to join us for a 4-day course on our campus in Ann Arbor from May 21-24. The MINT Workshop will be heavily subsidized by the NSF. Participants will only be responsible for the transportation costs and their dinners. Registration and supplies are graciously provided by NSF NeuroNex

Registration will close on March 16, 2018. If demand exceeds the class size then positions will be selected by the instructors based on the applicants prior experience and intended usage. Please briefly describe your experience and broad experimental use (no confidential details please). Our shared vision is to disseminate as many of these technologies conjointly for the acceleration of neuroscience. A final decision will be emailed to all by March 23, 2018.

 

CLARITY and systemic AAVs

Instructors: Viviana Gradinaru, Jennifer Treweek

Lesson goals:

  • Theory and practice of tissue clearing (brain and skull) using CLARITY and variants
  • Label of tissue in conjunction with tissue clearing, including IHC, protein, and RNA labeling
  • Theory and hands-on imaging of tissue cleared samples, including 1 hr of confocal time
  • Theory and practice of AAVs that can be delivered systemically with novel caspids, e.g. AAV-PHP.B and -PHP.eB

Reconstructing neural circuits by labeling, imaging and tracing Brainbow samples

Instructor: Dawen Cai

Lesson goals:

  • Principles of stochastic multispectral labeling of the nervous system
  • Understand Brainbow sample preparation
  • Theory and practice of Brainbow 3D imaging, including 2 or 3 hrs of confocal time
  • Post-acquisition image processing and neuronal tracing of Brainbow images

Optogenetic Experimentation Using a uLED Optoelectrode

Instructors: Kamran DibaMisi Voeroeslakos

Lesson goals:

  • Prerequisites: stereotactic surgery
  • Hands-on optoelectrode preparation for chronic surgery (designing and using microdrives)
  • Methods with optoelectrodes, including cell-type identification and noise/artifact reduction
  • Hands-on surgical experience using a chronic uLED array with simultaneous multi-channel stimulation and recording

Microelectrode array design and measurement

Instructors: James Weiland, John Seymour

Lesson goals:

  • Understanding of high-density electrode recording/stimulation arrays including electrochemical impedance spectroscopy, cyclic voltammetry, and electrode materials
  • Hands-on characterization of high surface area platinum electrodes, including charge balanced stimulation techniques
  • Review of software options for high-density polytrode spike sorting

Note: The training workshop is intended for experienced graduate students, or postdocs and PIs. Please send questions to ContactMINT@umich.edu.