Electrical Engineering and Computer Science

AAVs

The Gradinaru Lab has developed an AAV capable of systemic transfection. Recently, the first AAV for widespread systemic use was AAV-PHP.B and published in Nature. Newer variants have also been validated and shared in a Nature Protocols article that includes protocols for production, purification, and administration. AAV-PHP and its variants can be accessed at the Clover Center at Caltech. Since the Clover Center has limited supplies, future plans include disseminating via the Michigan Vector Core

If needed for your work, other related plasmids including our capsids, are deposited at Addgene here.  Addgene now provides PHP.eB viruses. PHP.S viruses are coming soon!