Our MISSION is to disseminate these technologies even while we further develop and validate them. NeuroNex MINT is looking for neuroscience labs who can combine multiple technologies to advance structure-function analysis for brain mapping. Please contact us to learn how you can combine the platforms below to improve your methodology. Our team is providing an annual MINT Training Workshop to instruct neuroscientists on how best to use each technology.
Click below to download DATASHEETS and see if these research tools can be used in your lab.
The Gradinaru Lab has developed an AAV capable of systemic transfection. Recently, the first AAV for widespread systemic use was AAV-PHP.B and published in Nature. Newer variants have also been validated and shared on BioRXiv. including the protocol for production, purification, and administration. AAV-PHP and its variants can be accessed at the Clover Center at Caltech. Since the Clover Center has limited supplies, future plans include disseminating via the Michigan Vector Core. If needed for your work, other related plasmids including our capsids, are deposited at Addgene here. Addgene now provides PHP.eB viruses. PHP.S viruses are coming soon!
Brainbow and XFP
Dawen Cai is a pioneering researcher of Brainbow and continues to improve its usefulness and accessibility. The Cai Lab is looking for new collaborators to develop novel means to deliver and control Brainbow expression. The Cre version of Brainbow with AAV9 is currently available at Addgene. Similarly, XFP fluorescent proteins also achieve random color mixing with CNS neurons. If you hope to use XFP labeling with tissue clearing, please also contact the Clover Center at Caltech.
Carbon microthreads have many benefits including chemical sensing and minimal tissue damage. The Chestek Lab continues to develop higher density versions of carbon threads and can distribute these on a limited basis to the community. Click here for more information about this technology.
The goal of this platform is to provide a coating service for any substrate (wire, carbon thread, silicon, or polymer) using a novel electroplating method developed by Jim Weiland and colleagues at Platinum Group Coatings.
High-density optoelectrodes developed in the Yoon Lab are NOW being disseminated. These are single color only and the specifications are provided in the following document. (DATASHEET: PassiveOptoGen1Datasheet_V1.8) Please let us know if there is a future configuration required for your neuroscience experiment. Request samples.
Two new things to share too…We now have a FAQ list inside of a Google Group called Michigan-Optoelectrodes. Also, check out our surgery video showing how to use the microdrive and general surgery techniques with a uLED.
The Gradinaru Lab continues to develop methods to improve the speed of Clarity and its compatibility with existing immunohistochemistry. This technique is published in Nature Protocols and an abbreviated troubleshooting table can be found here: Troubleshooting Clarity Table 5.
The Cai Lab has developed an ImageJ plugin called nTracer to semi-autonomously trace circuits. This is available to download, along with the manual, a sample image, sample tracing results, and tutorial videos here.