Our MISSION is to disseminate these technologies even while we further develop and validate them. NeuroNex MINT is looking for neuroscience labs who can combine multiple technologies to advance structure-function analysis for brain mapping. Please contact us to learn how you can combine the platforms below to improve your methodology. Our team is providing an annual MINT Training Workshop to instruct neuroscientists on how best to use each technology.
Click below to download DATASHEETS and see if these research tools can be used in your lab.
Optoelectrodes with no artifact and high temporal resolution are available via our sample request form. These are silicon neural probes with 12 blue micro-LEDs and 32 PtIr electrodes. Please see our datasheet for complete specifications, details on our open-source micro-LED driver (OSC1Lite), some FAQ, and a surgery video.
The Gradinaru Lab has developed an AAV capable of systemic transfection. Recently, the first AAV for widespread systemic use was AAV-PHP.B and published in Nature. Newer variants have also been validated and shared in a Nature Protocols article that includes protocols for production, purification, and administration. AAV-PHP and its variants can be accessed at the Clover Center at Caltech. Since the Clover Center has limited supplies, future plans include disseminating via the Michigan Vector Core. If needed for your work, other related plasmids including our capsids, are deposited at Addgene here. Addgene now provides PHP.eB viruses. PHP.S viruses are coming soon!
Brainbow and XFP
Dawen Cai is a pioneering researcher of Brainbow and continues to improve its usefulness and accessibility. The Cai Lab is looking for new collaborators to develop novel means to deliver and control Brainbow expression. The Cre version of Brainbow with AAV9 is currently available at Addgene. The Brainbow antibody is also now available through Ximbio, which improves the signal amplitude of your Brainbow labeled tissue. Similarly, XFP fluorescent proteins also achieve random color mixing with CNS neurons. If you hope to use XFP labeling with tissue clearing, please also contact the Clover Center at Caltech.
Carbon fiber electrodes have many benefits including chemical sensing and minimal tissue damage. The Chestek Lab continues to develop higher density versions of carbon fibers and can distribute these on a limited basis to the community. Click here for more information about this technology.
The goal of this platform is to provide a coating service for any substrate (wire, carbon fiber, silicon, or polymer) using a novel electroplating method developed by Jim Weiland and colleagues at Platinum Group Coatings.
The Gradinaru Lab continues to develop methods to improve the speed of Clarity and its compatibility with existing immunohistochemistry. This technique is published in Nature Protocols and an abbreviated troubleshooting table can be found here: Troubleshooting Clarity Table 5.
The Cai Lab has developed an ImageJ plugin called nTracer to semi-autonomously trace circuits. This is available to download, along with the manual, a sample image, sample tracing results, and tutorial videos here. A recent article in Bioinformatics describes the software and methods used to accomplish multispectral tracing with Brainbow.